The Core facilities and faculty provide extensive analytical capabilities for pharmacology research, including methods for most currently available antiretroviral compounds and numerous drugs used for HIV complications. Advanced assays include quantitation of intracellular triphosphates for nucleoside analogues, quantitation of drugs in peripheral tissues such as the cerebrospinal fluid and quantitation of unbound drug concentrations. State-of-the-art highly sensitive methods are developed using liquid chromatography/tandem mass spectroscopy.
Investigators are encouraged to contact the Core Director to discuss their projects and aims.
Drug Family | Drug | Technique | Matrix |
---|---|---|---|
* In development | |||
Nucleoside/ Nucleotide Analogues |
ddI | HPLC-UV^ | plasma |
3′-TC | HPLC-UV | plasma | |
ZDV & ZDV-glucuronide | HPLC-UV | plasma | |
ZDV & ZDV-glucuronide | HPLC-UV | urine | |
ZDV & ZDV-mono-, di-, triphosphate | Solid phase extraction/LC/Tandem MS | PBMCs | |
d4T -TP | LC/Tandem MS | PBMCs | |
abacavir | HPLC-UV | Plasma | |
ddATP* | LC/Tandem MS | PBMCs | |
Endogenous nucleotide (NRTI competitor) | thymidine triphosphate | LC/Tandem MS | PBMCs |
Nonnucleoside Reverse Transcriptase Inhibitors (NNRTI) | nevirapine | HPLC-UV | plasma |
nevirapine | LC/Tandem MS | plasma | |
Protease Inhibitors (PI) | amprenavir | LC/Tandem MS | plasma |
indinavir | LC/Tandem MS | plasma | |
nelfinavir & M8 | LC/Tandem MS | plasma | |
nelfinavir & M8 | LC/Tandem MS | CSF | |
ritonavir | LC/Tandem MS | plasma | |
saquinavir | LC/Tandem MS | plasma | |
Oligonucleotide | Zintevir | HPLC-UV | plasma |
Antivirals | thalidomide | HPLC-UV | plasma |
acyclovir | HPLC-UV | plasma | |
foscarnet | HPLC-EC | plasma | |
ganciclovir | HPLC-FLU | plasma | |
ganciclovir | HPLC-UV | urine | |
ribavirin | HPLC-UV | whole blood | |
Other Compounds | cortisol & 6-beta-hydroxycortisol | HPLC-UV | urine |
cefpodixome | HPLC-UV | plasma | |
ceftizoxime | HPLC-UV | plasma | |
ticarcillin | HPLC-UV | plasma | |
Iothalamate & PAH | HPLC-UV | plasma | |
Iothalamate & PAH | HPLC-UV | urine | |
GMCSF | ELISA | serum |